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Posts Tagged ‘DNA extraction’

A common lab practice in 4ESO is the DNA Extraction, before doing it in the laboratory it could be good to check this virtual practice.

In this slide presentation the process of DNA extraction is explained to make you understand its  scientific basis:

If you prefer watching a video, you can check this bilingual explanation of the lab practice.

May be you are wondering why did we add soap or detergent? or why did we add pineapple juice or tenderizer?

You can get a pdf script and more information from this excellent link:

Image by Jokulhaupar

And here by, you have a quick script to do this lab practice (based on this other one)

Home made DNA Extraction- Easy  Protocol:

  • Smash your organic sample until you get a soupy mixture. What we are going to want to use is the homogeneous solution in the middle. You probably won’t need all of this but it’s good to have extra just in case.
  • Add one tablespoonful of dish washing detergent (or soap) and a pinch of salt (NaCl) to the above solution. Slowly stir this for five minutes.
  • Add some drops of pineapple juice (or a pinch meat tenderizer) to the above solution. Again, stir for five minutes.
  • Pour and filter enough of the solution into your container to a level of two inches or so. Pour slowly out of the blender so you get the middle layer of solution.
  • Drizzle (Tilt the container and pour SLOWLY down the inside of the glass) cold alcohol into the above container. If you do this correctly, a clear layer of alcohol will be floating on top of the denser split pea solution. Add enough alcohol so there is a three inch layer above the split pea solution.
  • Now, let the container sit and observe the alcohol layer.


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Illustration by 
Michael Dunning, found here

Talking about applied genetics or Genetic Engineering, I must absolutely reccomend this web page. Genetic Science Learning Center (2010, March 14) Teach.Genetics (TM). Learn.Genetics. Retrieved March 14, 2010, from http://teach.genetics.utah.edu/

To review the main concepts about genetics that we have studied you can follow this tour of the basics to answer the essential questions as What is a gene? What is DNA? What is a Chromosone? What is a trait? What is a protein? and what is Heredity?

You can try  making a karyotype and here you can build a DNA molecule

Then I recommend you these games or interactive activities about genetic engineering:


  • For example, about cloning

WHAT IS CLONING ?

CLICK AND CLONE

IS IT CLONING ?  OR NOT ?

  • About Stem Cells:

WHAT IS A STEM CELL ?

WHAT ARE SOME DIFFERENT TYPES OF STEM CELLS ?

  • About gene therapy:

SPACE DOCTOR you sohuld also check (TOOLS OF THE TRADE)

CYSTIC FIBROSIS: CASE STUDY

  • How  to make GMOs

Transgenic manipulation

  • About genetic engineering lab technics:

PCR Virtual Lab

Gel Electrophoresis Virtual Lab

DNA Extraction Virtual Lab

DNA Microaarray Virtual Lab

  • An also about such an interesting subject as Epigenetics:

THE EPIGENOME AT A GLANCE

GENE CONTROL

From different web sites you can also check this  flash  explanation about how to clone a gene or another similar one here where restriction enzymes action is well explained.

Hereby a puzzle game plus information  to understand what GMOs are and how they are obtained.

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